Summary
Type: SPE
Application #: 305790
Catalog#: 730012 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 200mg, 50/pk
Detection:
Conditions: Column conditioning: 3 mL methanol, then 3 mL water
(Do not let run the column dry.)
Sample aspiration: each water sample (volume: 500 mL) was percolated through the column by regulated vacuum (flow: 5–10 mL/min)
Drying: by centrifugation (2500 rpm for 2 min) and then by vacuum (10 min)
Elution: by centrifugation (2500 rpm for 2 min) with 1 mL methanol and then with 1 mL dichloromethane
Evaporation: to about 100 µL with nitrogen gas (40 °C)
Reconstitution: 200 µL acetonitrile
As internal standard PCB 153 was added. Samples were stored in the dark at about -20 °C until GC-MS analysis.
Authors: Kouzayha, A. et al.
Source: American J Analytical Chemistry, 2012, 3, 257–26